Yes, but very little. My stance is (for most instances) is that if the surfaces are cleaned and straight, a gasket should seal properly without additional "goop".
Although, there are places that will benefit from some use of "goop". The trick is to know how to use goop sparingly, and have clean and straight surfaces to start with.
Remember that the "goop" that squeezes out is generally the same amount that squeezes into your engine too.
There are also many different types of "goop" and many have different applications.